Glomerular disease has a tendency to progress to chronic renal failure after initial injury. There is no method to prevent or improve the progression of renal disease except minimal change nephrotic syndrome which can be improved by steroid
therapy. The
progresson of renal disease can be identified as a glomerulosclerosis by histological examination, and it is known that a mesangial cell has a significant pathogenic role in the protression of glomerulosclerosis. The basic mechanism of
glomerulosclerosis is the proliferation and the collagen synthesis of mesangial cells.
In this study, after in vitro culture of rat mesangial cells, the proliferation and the collagen synthesis of mesangial cells were measred. As factors which can effect on cultured rat mesangial cells. Low density lipoprotein (LDL) was chosen
because
glomerulosclerosis is histologically similar to atherosclerosis of which LDL is considered as a risk factor and in many other cells.
Sprague-dawley rats (age of 4~6 weeks) were sacriliced to culture mesangial cells in vitro. LDL was isolated from human blood by ultraentrifuge using the difference of density of lipoproteins. Mesangial cells were identified by indirect
immunofluorescent method and by the fact that they could grow in D-valine media and be resistant to puromycin. The isolated LDL was confirmed y lipid electrophoresis.
The results were as follows: At a concentration of 50 ug/ml. 100 ug/ml. 150 ug/ml, 200 ug/ml of LDL, the H-thymidine uptake was increased to 138±23%, 171±39%, 226±58%, 214±38% when compared to the control value. The 3H-proline uptake did not
increase at the concentration of 50 ug/ml, 100 ug/ml of LDL but increased to 182±10%, 143±32% at the concentration of 150 ug/ml, 200 ug/ml when compared to the control value.
In conclusion, hyperlipidemia plays a significant role in the pathogenesis of glomerulosclerosis.
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